Legionella 5S rRNA PCR melting temperature analysis discriminates high-risk species associated with disease severity

ABSTRACT Legionella species are important causes of community-acquired pneumonia. The diagnostic performance and clinical implications of a fluorescence resonance energy transfer (FRET)-based real-time PCR assay targeting the Legionella 5S rRNA gene were evaluated, with emphasis on melting temperatu...

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Autori principali:	Aaron M. Pulsipher, Georges Khattar, Emily Harris, Valerie White, Camron Stout, Holenaraspur R. Vikram, Robin Patel, Patricia J. Simner
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology 2026-07-01
Serie:	Journal of Clinical Microbiology
Soggetti:	clinical outcomes melting temperature PCR diagnostics Legionella
Accesso online:	https://journals.asm.org/doi/10.1128/jcm.00356-26
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