Increasing quality, throughput and speed of sample preparation for strand-specific messenger RNA sequencing

Abstract Background RNA-Sequencing (RNA-seq) is now commonly used to reveal quantitative spatiotemporal snapshots of the transcriptome, the structures of transcripts (splice variants and fusions) and landscapes of expressed mutations. However, standard approaches for library construction typically r...

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Autors principals:	Simon Haile, Richard D. Corbett, Tina MacLeod, Steve Bilobram, Duane Smailus, Philip Tsao, Heather Kirk, Helen McDonald, Pawan Pandoh, Miruna Bala, Martin Hirst, Diane Miller, Richard A. Moore, Andrew J. Mungall, Jacquie Schein, Robin J. Coope, Yussanne Ma, Yongjun Zhao, Rob A. Holt, Steven J. Jones, Marco A. Marra
Format:	Artigo
Idioma:	Inglês
Publicat:	BMC 2017-07-01
Col·lecció:	BMC Genomics
Matèries:	Ampure XP magnetic beads Next-generation sequencing Library construction Strand-specific dUTP Uracil DNA N-Glycosylase
Accés en línia:	http://link.springer.com/article/10.1186/s12864-017-3900-6
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