Protocol for generating splice isoform-specific mouse mutants using CRISPR-Cas9 and a minigene splicing reporter

Summary: Here, we present a protocol to alter the production of alternatively spliced mRNA variants, without affecting the overall gene expression, through CRISPR-Cas9-engineered genomic mutations in mice. We describe steps for designing guide RNA to direct Cas9 endonuclease to consensus splice site...

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Главные авторы:	Yudong Teng, Kelsey Arbogast, Harald Junge, Zhe Chen
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Elsevier 2025-03-01
Серии:	STAR Protocols
Предметы:	CRISPR Developmental biology Model Organisms Molecular Biology Neuroscience
Online-ссылка:	http://www.sciencedirect.com/science/article/pii/S2666166724007081
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