FISHing for ciliates: Catalyzed reporter deposition fluorescence in situ hybridization for the detection of planktonic freshwater ciliates

Planktonic ciliate species form multiple trophic guilds and are central components of freshwater food webs. Progress in molecular analytical tools has opened new insight into ciliate assemblages. However, high and variable 18S rDNA copy numbers, typical for ciliates, make reliable quantification by...

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Detalhes bibliográficos

Principais autores:	Gianna Dirren-Pitsch, Dominique Bühler, Michaela M. Salcher, Barbara Bassin, Alizée Le Moigne, Martina Schuler, Jakob Pernthaler, Thomas Posch
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Frontiers Media S.A. 2022-12-01
coleção:	Frontiers in Microbiology
Assuntos:	planktonic ciliates CARD-FISH fluorescence in situ hybridization lake water samples quantitative protargol staining ciliate quantification
Acesso em linha:	https://www.frontiersin.org/articles/10.3389/fmicb.2022.1070232/full
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