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Combined Locked Nucleic Acid Probes and High-Resolution Melting Curve Analysis for Detection of Rifampicin-Resistant Tuberculosis in Northern Thailand

Rifampicin-resistant tuberculosis (RR-TB) has become a major threat globally. This study aims to develop a new assay, RIF-RDp, to enhance the detection of RR-TB based on combined locked nucleic acid (LNA) probes with high-resolution melting curve analysis (HRM). Two new LNA probes were designed to t...

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Main Authors: Yee Mon Thant, Sukanya Saikaew, Chayada Sitthidet Tharinjaroen, Ponrut Phunpae, Rodjana Pongsararuk, Kanya Preechasuth, Bordin Butr-Indr, Sorasak Intorasoot, Khajornsak Tragoolpua, Angkana Chaiprasert, Usanee Wattananandkul
Format: Artigo
Language:Inglês
Published: MDPI AG 2022-09-01
Series:Diagnostics
Subjects:
tuberculosis
diagnosis
molecular test
real time PCR
single nucleotide polymorphism
Online Access:https://www.mdpi.com/2075-4418/12/10/2307
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