Mismatch-Enhanced Specific PCR (MES-PCR): A Rapid and Cost-Effective Method for Screening CRISPR/Cas9-Induced Mutations

CRISPR-associated protein 9 (Cas9)-mediated editing generates numerous mutations. Existing detection methods, such as ACT-PCR, T7EI endonuclease cleavage, HRM analysis, and high-throughput sequencing, often require stringent conditions, expensive reagents, or specialized instruments. Here, we introd...

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Autors principals:	Peng Tian, Bengang Yao, Wenjing Lin, Maoting Yuan, Shuran Li, Yuzhu Qin, Shuang Chen, Tao Lai, Zhenbiao Yang, Wenwei Lin, Xiang Zhou
Format:	Artigo
Idioma:	Inglês
Publicat:	MDPI AG 2026-07-01
Col·lecció:	Biology
Matèries:	ACT-PCR MES-PCR mutation CRISPR/Cas9 genotyping
Accés en línia:	https://www.mdpi.com/2079-7737/15/13/1089
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