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The 28S rRNA RT-qPCR assay for host depletion evaluation to enhance avian virus detection in Illumina and Nanopore sequencing

Abundant host and bacterial sequences can obscure the detection of less prevalent viruses in untargeted next-generation sequencing (NGS). Efficient removal of these non-targeted sequences is vital for accurate viral detection. This study presents a novel 28S ribosomal RNA (rRNA) RT-qPCR assay design...

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書誌詳細
主要な著者: Iryna V. Goraichuk, Mark Harden, Erica Spackman, David L. Suarez
フォーマット: Artigo
言語:Inglês
出版事項: Frontiers Media S.A. 2024-01-01
シリーズ:Frontiers in Microbiology
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オンライン・アクセス:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1328987/full
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