क्यूआर कोड

2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

Abstract Transcription of eukaryotic genomes involves complex alternative processing of RNAs. Sequencing of full-length RNAs using long reads reveals the true complexity of processing. However, the relatively high error rates of long-read sequencing technologies can reduce the accuracy of intron ide...

पूर्ण विवरण

में बचाया:
मुख्य लेखकों: Matthew T. Parker, Katarzyna Knop, Geoffrey J. Barton, Gordon G. Simpson
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: BMC 2021-03-01
श्रृंखला:Genome Biology
विषय:
Splicing
Long-read sequencing
Spliced alignment
RNA-seq
Gene expression
Transcriptome assembly
ऑनलाइन पहुंच:https://doi.org/10.1186/s13059-021-02296-0
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