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Cloning, expression, and analysis of the group 2 allergen from Dermatophagoides farinae from China

To obtain the recombinant group 2 allergen product of Dermatophagoides farinae (Der f 2), the Der f 2 gene was synthesized by RT-PCR. The full-length cDNA comprised 441 nucleotides and was 99.3% identical to the reference sequence (GenBank AB195580). The cDNA was bound to vector pET28a to construct...

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Publié dans:Anais da Academia Brasileira de Ciências
Auteurs principaux: Cui Yu-bao, Ying Zhou, Shi Weihong, Ma Guifang, Li Yang, Wang Yungang
Format: Artigo
Langue:Inglês
Publié: Academia Brasileira de Ciências 2010
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Accès en ligne:https://www.redalyc.org/articulo.oa?id=32717686017
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