Cloning of the Bovine Immunodeficiency Virus gag Gene and Development of a Recombinant-Protein-Based Enzyme-Linked Immunosorbent Assay

An enzyme-linked immunosorbent assay (ELISA) was established for the rapid detection of specific bovine immunodeficiency virus (BIV) antibodies in cattle, using recombinant Gag protein as an antigen. The gag coding region from BIV was cloned into an expression vector, pQE32, which expressed high lev...

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Bibliographic Details
Main Authors: Zheng, Ling, Swanson, Michelle, Liao, Jinghua, Wood, Charles, Kapil, Sanjay, Snider, Ron, Loughin, Thomas A., Minocha, Harish C.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2000
Subjects:
Microbial Immunology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC95913/
https://ncbi.nlm.nih.gov/pubmed/10882651
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