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Efficient System for Directed Integration into the Lactobacillus acidophilus and Lactobacillus gasseri Chromosomes via Homologous Recombination

An efficient method is described for the generation of site-specific chromosomal integrations in Lactobacillus acidophilus and Lactobacillus gasseri. The strategy is an adaptation of the lactococcal pORI system (K. Leenhouts, G. Venema, and J. Kok, Methods Cell Sci. 20:35–50, 1998) and relies on the...

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Autors principals: Russell, W. M., Klaenhammer, T. R.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2001
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC93172/
https://ncbi.nlm.nih.gov/pubmed/11526048
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.9.4361-4364.2001
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