Differentiation of Methanosaeta concilii and Methanosarcina barkeri in Anaerobic Mesophilic Granular Sludge by Fluorescent In Situ Hybridization and Confocal Scanning Laser Microscopy

Oligonucleotide probes, designed from genes coding for 16S rRNA, were developed to differentiate Methanosaeta concilii, Methanosarcina barkeri, and mesophilic methanogens. All M. concilii oligonucleotide probes (designated MS1, MS2, and MS5) hybridized specifically with the target DNA, but MS5 was t...

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Hauptverfasser: Rocheleau, Sylvie, Greer, Charles W., Lawrence, John R., Cantin, Christiane, Laramée, Louise, Guiot, Serge R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 1999
Schlagworte:
General Microbial Ecology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC91320/
https://ncbi.nlm.nih.gov/pubmed/10224023
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