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Optimized protocol for tRNA identification in the ribosomal complexes from human cell lines
Here, we describe a protocol for tRNA identification in the 60S ribosome-nascent peptide complex co-purified with Nuclear Export Mediator Factor (NEMF), a responsible factor for C-terminal alanine and threonine tailing of the nascent peptide. Our protocol is based on regular reverse transcription fo...
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| 發表在: | STAR Protoc |
|---|---|
| Main Authors: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Elsevier
2021
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8220392/ https://ncbi.nlm.nih.gov/pubmed/34189478 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100615 |
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