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Combining transposon mutagenesis and reporter genes to identify novel regulators of the topA promoter in Streptomyces

BACKGROUND: Identifying the regulatory factors that control transcriptional activity is a major challenge of gene expression studies. Here, we describe the application of a novel approach for in vivo identification of regulatory proteins that may directly or indirectly control the transcription of a...

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Bibliografiske detaljer
Udgivet i:Microb Cell Fact
Main Authors: Gongerowska-Jac, Martyna, Szafran, Marcin Jan, Jakimowicz, Dagmara
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8120823/
https://ncbi.nlm.nih.gov/pubmed/33985526
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-021-01590-7
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