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Characterizing and circumventing sequence restrictions for synthesis of circular RNA in vitro
Just as eukaryotic circular RNA (circRNA) is a product of intracellular backsplicing, custom circRNA can be synthesized in vitro using a transcription template in which transposed halves of a split group I intron flank the sequence of the RNA to be circularized. Such permuted intron–exon (PIE) const...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8034654/ https://ncbi.nlm.nih.gov/pubmed/33406226 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa1256 |
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