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Characterizing and circumventing sequence restrictions for synthesis of circular RNA in vitro

Just as eukaryotic circular RNA (circRNA) is a product of intracellular backsplicing, custom circRNA can be synthesized in vitro using a transcription template in which transposed halves of a split group I intron flank the sequence of the RNA to be circularized. Such permuted intron–exon (PIE) const...

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Bibliografiska uppgifter
I publikationen:Nucleic Acids Res
Huvudupphovsmän: Rausch, Jason W, Heinz, William F, Payea, Matthew J, Sherpa, Chringma, Gorospe, Myriam, Le Grice, Stuart F J
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2021
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC8034654/
https://ncbi.nlm.nih.gov/pubmed/33406226
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa1256
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