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Gene Transfection of Primary Mouse Hepatocytes in 384-Well Plates
We report the development of an in vitro transfection assay to test the efficiency of non-viral vector DNA nanoparticle transfection of primary hepatocytes. The protocol describes the isolation of viable hepatocytes from a mouse by collagenous perfusion. Primary mouse hepatocytes are plated in 384-w...
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| Izdano u: | Anal Biochem |
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| Glavni autori: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2022
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7936984/ https://ncbi.nlm.nih.gov/pubmed/32910973 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2020.113911 |
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