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Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

A novel TaqMan 5‐plex real‐time PCR using a combination of locked nucleic acid‐modified (LNA)‐ and minor groove binding (MGB)‐conjugated DNA probes was developed for identification and differentiation between the four main pathogenic Brachyspira species in swine. B. hyodysenteriae, B. pilosicoli, an...

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Publicat a:Microbiologyopen
Autors principals: Scherrer, Simone, Stephan, Roger
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2021
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7887428/
https://ncbi.nlm.nih.gov/pubmed/33650802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/mbo3.1169
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