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Screening natural product extracts for potential enzyme inhibitors: protocols, and the standardisation of the usage of blanks in α-amylase, α-glucosidase and lipase assays

BACKGROUND: Enzyme assays have widespread applications in drug discovery from plants to natural products. The appropriate use of blanks in enzyme assays is important for assay baseline-correction, and the correction of false signals associated with background matrix interferences. However, the blank...

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Detalhes bibliográficos
Publicado no:Plant Methods
Main Authors: Lankatillake, Chintha, Luo, Shiqi, Flavel, Matthew, Lenon, George Binh, Gill, Harsharn, Huynh, Tien, Dias, Daniel Anthony
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2021
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7789656/
https://ncbi.nlm.nih.gov/pubmed/33407662
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-020-00702-5
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