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Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer

As our understanding of soil biology deepens, there is a growing demand for investigations addressing microbial processes in the earth beneath the topsoil layer, called subsoil. High clay content in subsoils often hinders the recovery of sufficient quantities of DNA as clay particles bind nucleic ac...

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Vydáno v:Microorganisms
Hlavní autoři: Guerra, Victor, Beule, Lukas, Lehtsaar, Ena, Liao, Hui-Ling, Karlovsky, Petr
Médium: Artigo
Jazyk:Inglês
Vydáno: MDPI 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7232467/
https://ncbi.nlm.nih.gov/pubmed/32272709
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/microorganisms8040532
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