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Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer

As our understanding of soil biology deepens, there is a growing demand for investigations addressing microbial processes in the earth beneath the topsoil layer, called subsoil. High clay content in subsoils often hinders the recovery of sufficient quantities of DNA as clay particles bind nucleic ac...

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Detalhes bibliográficos
Publicado no:Microorganisms
Main Authors: Guerra, Victor, Beule, Lukas, Lehtsaar, Ena, Liao, Hui-Ling, Karlovsky, Petr
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7232467/
https://ncbi.nlm.nih.gov/pubmed/32272709
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/microorganisms8040532
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