Efficient knock-in at the chicken ovalbumin locus using adenovirus as a CRISPR/Cas9 delivery system

In this study, efficient knock-in (KI) of human epidermal growth factor (hEGF) cDNA at the ovalbumin (OV) locus in cultured chicken cells was achieved using adenovirus as a delivery for CRISPR/Cas9 elements and optimizing donor vector construction. The strategy of recruiting donor DNA to the inserti...

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Publicat a:3 Biotech
Autors principals: Qin, Xiaolian, Xiao, Ning, Xu, Yu, Yang, Fengshuo, Wang, Xiaoli, Hu, Hao, Liu, Qingyou, Cui, Kuiqing, Tang, Xiaochuan
Format: Artigo
Idioma:Inglês
Publicat: Springer International Publishing 2019
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Original Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6861409/
https://ncbi.nlm.nih.gov/pubmed/31832301
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-019-1966-3
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