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High-fidelity amplified FISH for the detection and allelic discrimination of single mRNA molecules
Amplification of signals by the hybridization chain reaction (HCR) is a powerful approach for increasing signal strength in single-molecule fluorescence in situ hybridization, but probes tagged with an HCR initiator sequence are prone to producing false signals. Here we describe a system of interact...
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| 出版年: | Proc Natl Acad Sci U S A |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
2019
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6628817/ https://ncbi.nlm.nih.gov/pubmed/31221755 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1814463116 |
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