A urinary extracellular vesicle microRNA biomarker discovery pipeline; from automated extracellular vesicle enrichment by acoustic trapping to microRNA sequencing

Development of a robust automated platform for enrichment of extracellular vesicles from low sample volume that matches the needs for next-generation sequencing could remove major hurdles for genomic biomarker discovery. Here, we document a protocol for urinary EVs enrichment by utilizing an automat...

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Bibliographic Details
Published in:PLoS One
Main Authors: Ku, Anson, Ravi, Naveen, Yang, Minjun, Evander, Mikael, Laurell, Thomas, Lilja, Hans, Ceder, Yvonne
Format: Artigo
Language:Inglês
Published: Public Library of Science 2019
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6541292/
https://ncbi.nlm.nih.gov/pubmed/31141544
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0217507
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