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Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

A comprehensive identification of RNA-binding proteins (RBPs) is key to understanding the posttranscriptional regulatory network in cells. A widely used strategy for RBP capture exploits the polyadenylation [poly(A)] of target RNAs, which mostly occurs on eukaryotic mature mRNAs, leaving most bindin...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Huang, Rongbing, Han, Mengting, Meng, Liying, Chen, Xing
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235571/
https://ncbi.nlm.nih.gov/pubmed/30394395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58580
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