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Synthesis, characterization and function of an RNA-based transfection reagent

A synthetic 8-mer amphipathic trans-acting poly-2′-O-methyluridylic thiophosphate triester RNA element (2′-OMeUtaPS) mediates an efficient delivery of uncharged polyA-tailed phosphorodiamidate morpholino sequences (PMO) to HeLa pLuc 705 cells or to myotube muscle cells. The transfected antisense PMO...

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Bibliographic Details
Published in:Curr Protoc Nucleic Acid Chem
Main Authors: Jain, Harsh V., Boehler, Jessica F., Nagaraju, Kanneboyina, Beaucage, Serge L.
Format: Artigo
Language:Inglês
Published: 2018
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6020023/
https://ncbi.nlm.nih.gov/pubmed/29927123
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpnc.51
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