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Excision of Nucleopolyhedrovirus Form Transgenic Silkworm Using the CRISPR/Cas9 System

The CRISPR/Cas9-mediated genome engineering has been shown to efficiently suppress infection by disrupting genes of the pathogen. We recently constructed transgenic lines expressing CRISPR/Cas9 and the double sgRNA target Bombyx mori nucleopolyhedrovirus (BmNPV) immediate early-1 (ie-1) gene in the...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Dong, Zhanqi, Dong, Feifan, Yu, Xinbo, Huang, Liang, Jiang, Yaming, Hu, Zhigang, Chen, Peng, Lu, Cheng, Pan, Minhui
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5820291/
https://ncbi.nlm.nih.gov/pubmed/29503634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2018.00209
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