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A new nonradioactive deoxyhypusine synthase assay adaptable to high throughput screening

Deoxyhypusine synthase (DHS) catalyzes the post-translational modification of eukaryotic translation factor 5A (eIF5A) by the polyamine, spermidine, that converts one specific lysine residue to deoxyhypusine [N(ε)-4-aminobutyl(lysine)], which is subsequently hydroxylated to hypusine [N(ε)-4-amino-2-...

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Publicat a:Amino Acids
Autors principals: Park, Myung Hee, Mandal, Ajeet, Mandal, Swati, Wolff, Edith C.
Format: Artigo
Idioma:Inglês
Publicat: 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5693660/
https://ncbi.nlm.nih.gov/pubmed/28819816
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00726-017-2477-3
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