Effects of isoleucine 135 side chain length on the cofactor donor-acceptor distance within F(420)H(2):NADP(+) oxidoreductase: A kinetic analysis()

F(420)H(2):NADP(+) Oxidoreductase (Fno) catalyzes the reversible reduction of NADP(+) to NADPH by transferring a hydride from the reduced F(420) cofactor. Here, we have employed binding studies, steady-state and pre steady-state kinetic methods upon wtFno and isoleucine 135 (I135) Fno variants in or...

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Gepubliceerd in:Biochem Biophys Rep
Hoofdauteurs: Le, Cuong Quang, Oyugi, Mercy, Joseph, Ebenezer, Nguyen, Toan, Ullah, Md Hasmat, Aubert, Joshua, Phan, Thien, Tran, Joseph, Johnson-Winters, Kayunta
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2016
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Research Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5614548/
https://ncbi.nlm.nih.gov/pubmed/28955995
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2016.11.012
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