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CRISPR-mediated Integration of Large Gene Cassettes using AAV Donor Vectors
The CRISPR/Cas9 system has recently been shown to facilitate high levels of precise genome editing using adeno associated viral (AAV) vectors to serve as donor template DNA during homologous recombination (HR). However, the maximum AAV packaging capacity of ~4.5 kilobases limits the donor size. Here...
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| 發表在: | Cell Rep |
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| Main Authors: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2017
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5568673/ https://ncbi.nlm.nih.gov/pubmed/28723575 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2017.06.064 |
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