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Single-molecule imaging of the BAR-domain protein Pil1p reveals filament-end dynamics

Molecular assemblies can have highly heterogeneous dynamics within the cell, but the limitations of conventional fluorescence microscopy can mask nanometer-scale features. Here we adapt a single-molecule strategy to perform single-molecule recovery after photobleaching (SRAP) within dense macromolec...

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Bibliographic Details
Published in:Mol Biol Cell
Main Authors: Lacy, Michael M., Baddeley, David, Berro, Julien
Format: Artigo
Language:Inglês
Published: The American Society for Cell Biology 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5555653/
https://ncbi.nlm.nih.gov/pubmed/28659415
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E17-04-0238
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