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Efficient Genome Editing of a Facultative Thermophile Using Mesophilic spCas9
[Image: see text] Well-developed genetic tools for thermophilic microorganisms are scarce, despite their industrial and scientific relevance. Whereas highly efficient CRISPR/Cas9-based genome editing is on the rise in prokaryotes, it has never been employed in a thermophile. Here, we apply Streptoco...
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| 發表在: | ACS Synth Biol |
|---|---|
| Main Authors: | , , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Chemical
Society
2017
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| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5440800/ https://ncbi.nlm.nih.gov/pubmed/28146359 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.6b00339 |
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