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Comparison of two commercial broad-range PCR and sequencing assays for identification of bacteria in culture-negative clinical samples

BACKGROUND: Culturing has long been the gold standard for detecting aetiologic agents in bacterial infections. In some cases, however, culturing fails to detect the infection. To further investigate culture-negative samples, amplification and subsequent sequencing of the 16S rRNA gene is often appli...

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Publicado en:BMC Infect Dis
Main Authors: Stavnsbjerg, Camilla, Frimodt-Møller, Niels, Moser, Claus, Bjarnsholt, Thomas
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5368927/
https://ncbi.nlm.nih.gov/pubmed/28347280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12879-017-2333-9
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