A Robot-Based Platform to Measure Multiple Enzyme Activities in Arabidopsis Using a Set of Cycling Assays: Comparison of Changes of Enzyme Activities and Transcript Levels during Diurnal Cycles and in Prolonged Darkness

A platform has been developed to measure the activity of 23 enzymes that are involved in central carbon and nitrogen metabolism in Arabidopsis thaliana. Activities are assayed in optimized stopped assays and the product then determined using a suite of enzyme cycling assays. The platform requires in...

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Main Authors: Gibon, Yves, Blaesing, Oliver E., Hannemann, Jan, Carillo, Petronia, Höhne, Melanie, Hendriks, Janneke H.M., Palacios, Natalia, Cross, Joanna, Selbig, Joachim, Stitt, Mark
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Plant Biologists 2004
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC535875/
https://ncbi.nlm.nih.gov/pubmed/15548738
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1105/tpc.104.025973
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