Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting

OBJECTIVE: This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm...

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Bibliographische Detailangaben
Veröffentlicht in:Clin Exp Reprod Med
Hauptverfasser: Chi, Hee-Jun, Kwak, Su-Jin, Kim, Seok-Gi, Kim, Youn-Young, Park, Ji-Young, Yoo, Chang-Seok, Park, Il-Hae, Sun, Hong-Gil, Kim, Jae-Won, Lee, Kyeong-Ho
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Korean Society for Reproductive Medicine 2016
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Original Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5234288/
https://ncbi.nlm.nih.gov/pubmed/28090458
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5653/cerm.2016.43.4.199
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