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Notes and Tips for Improving Quality of Lipid-Protein Overlay Assays

To reduce costs of lipid-binding assays, allow for multiple lipids to be screened for protein binding simultaneously, and to make lipid binding more user friendly, lipids have been dotted onto membranes to investigate lipid-protein interactions. These assays are similar to a western blot where the m...

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Bibliographic Details
Published in:Anal Biochem
Main Authors: Shirey, Carolyn M., Scott, Jordan L., Stahelin, Robert V.
Format: Artigo
Language:Inglês
Published: 2016
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5097677/
https://ncbi.nlm.nih.gov/pubmed/27742211
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2016.10.009
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