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Evaluation of Lysis Methods for the Extraction of Bacterial DNA for Analysis of the Vaginal Microbiota
BACKGROUND: Recent studies on the vaginal microbiota have employed molecular techniques such as 16S rRNA gene sequencing to describe the bacterial community as a whole. These techniques require the lysis of bacterial cells to release DNA before purification and PCR amplification of the 16S rRNA gene...
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| Published in: | PLoS One |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Public Library of Science
2016
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5028042/ https://ncbi.nlm.nih.gov/pubmed/27643503 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0163148 |
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