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PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli
Efficiency and fidelity are the key obstacles for genome editing toolboxes. In the present study, a PCR-based tandem repeat assisted genome editing (TRAGE) method with high efficiency and fidelity was developed. The design of TRAGE is based on the mechanism of repair of spontaneous double-strand bre...
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| 出版年: | PLoS One |
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| 主要な著者: | , , , , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Public Library of Science
2016
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4809717/ https://ncbi.nlm.nih.gov/pubmed/27019283 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0149762 |
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