PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli

Efficiency and fidelity are the key obstacles for genome editing toolboxes. In the present study, a PCR-based tandem repeat assisted genome editing (TRAGE) method with high efficiency and fidelity was developed. The design of TRAGE is based on the mechanism of repair of spontaneous double-strand bre...

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Veröffentlicht in:PLoS One
Hauptverfasser: Liu, Yilan, Yang, Maohua, Chen, Jinjin, Yan, Daojiang, Cheng, Wanwan, Wang, Yanyan, Thygesen, Anders, Chen, Ruonan, Xing, Jianmin, Wang, Qinhong, Ma, Yanhe
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2016
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4809717/
https://ncbi.nlm.nih.gov/pubmed/27019283
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0149762
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