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CRISPRscan: designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo
CRISPR/Cas9 technology provides a powerful system for genome engineering. However, variable activity across different single guide RNAs (sgRNAs) remains a significant limitation. We have analyzed the molecular features that influence sgRNA stability, activity and loading into Cas9 in vivo. We observ...
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| 出版年: | Nat Methods |
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| 主要な著者: | , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2015
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4589495/ https://ncbi.nlm.nih.gov/pubmed/26322839 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3543 |
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