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A flexible and economical barcoding approach for highly multiplexed amplicon sequencing of diverse target genes

High throughput sequencing of phylogenetic and functional gene amplicons provides tremendous insight into the structure and functional potential of complex microbial communities. Here, we introduce a highly adaptable and economical PCR approach to barcoding and pooling libraries of numerous target g...

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Bibliographic Details
Published in:Front Microbiol
Main Authors: Herbold, Craig W., Pelikan, Claus, Kuzyk, Orest, Hausmann, Bela, Angel, Roey, Berry, David, Loy, Alexander
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2015
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4503924/
https://ncbi.nlm.nih.gov/pubmed/26236305
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2015.00731
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