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Structural and biochemical characterization of a metagenome-derived esterase with a long N-terminal extension

The genes encoding six novel esterolytic/lipolytic enzymes, termed LC-Est1∼6, were isolated from a fosmid library of a leaf-branch compost metagenome by functional screening using tributyrin agar plates. These enzymes greatly vary in size and amino acid sequence. The highest identity between the ami...

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Publicat a:Protein Sci
Autors principals: Okano, Hiroyuki, Hong, Xun, Kanaya, Eiko, Angkawidjaja, Clement, Kanaya, Shigenori
Format: Artigo
Idioma:Inglês
Publicat: Blackwell Publishing Ltd 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4282415/
https://ncbi.nlm.nih.gov/pubmed/25348365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2591
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