PCR Duplication: A One-Step Cloning-Free Method to Generate Duplicated Chromosomal Loci and Interference-Free Expression Reporters in Yeast

Here, we report on a novel PCR targeting-based strategy called ‘PCR duplication’ that enables targeted duplications of genomic regions in the yeast genome using a simple PCR-based approach. To demonstrate its application we first duplicated the promoter of the FAR1 gene in yeast and simultaneously i...

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Bibliographic Details
Published in:PLoS One
Main Authors: Huber, Florian, Meurer, Matthias, Bunina, Daria, Kats, Ilia, Maeder, Céline I., Štefl, Martin, Mongis, Cyril, Knop, Michael
Format: Artigo
Language:Inglês
Published: Public Library of Science 2014
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4262419/
https://ncbi.nlm.nih.gov/pubmed/25493941
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0114590
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