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Engineered protein A ligands, derived from a histidine-scanning library, facilitate the affinity purification of IgG under mild acidic conditions

BACKGROUND: In antibody purification processes, the acidic buffer commonly used to elute the bound antibodies during conventional affinity chromatograph, can damage the antibody. Herein we describe the development of several types of affinity ligands which enable the purification of antibodies under...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
मुख्य लेखकों: Tsukamoto, Masayuki, Watanabe, Hideki, Ooishi, Ayako, Honda, Shinya
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: BioMed Central 2014
विषय:
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC4107488/
https://ncbi.nlm.nih.gov/pubmed/25057290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1754-1611-8-15
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