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Multiplex genome editing by natural transformation
Editing bacterial genomes is an essential tool in research and synthetic biology applications. Here, we describe multiplex genome editing by natural transformation (MuGENT), a method for accelerated evolution based on the cotransformation of unlinked genetic markers in naturally competent microorgan...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
2014
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4066482/ https://ncbi.nlm.nih.gov/pubmed/24889608 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1406478111 |
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