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Multiplex genome editing by natural transformation

Editing bacterial genomes is an essential tool in research and synthetic biology applications. Here, we describe multiplex genome editing by natural transformation (MuGENT), a method for accelerated evolution based on the cotransformation of unlinked genetic markers in naturally competent microorgan...

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Autors principals: Dalia, Ankur B., McDonough, EmilyKate, Camilli, Andrew
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4066482/
https://ncbi.nlm.nih.gov/pubmed/24889608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1406478111
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