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Recombination Can Lead to Spurious Results in Retroviral Transduction with Dually Fluorescent Reporter Genes
Fluorescent proteins are routinely employed as reporters in retroviral vectors. Here, we demonstrate that transduction with retroviral vectors carrying a tandem-dimer Tomato (TdTom) reporter produces two distinct fluorescent cell populations following template jumping due to a single nucleotide poly...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3838247/ https://ncbi.nlm.nih.gov/pubmed/24067983 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.02524-13 |
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