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Acrylamide concentration determines the direction and magnitude of helical membrane protein gel shifts

SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which compris...

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Bibliografski detalji
Glavni autori: Rath, Arianna, Cunningham, Fiona, Deber, Charles M.
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2013
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3785759/
https://ncbi.nlm.nih.gov/pubmed/24019476
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1311305110
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