Intracellular Water Exchange for Measuring the Dry Mass, Water Mass and Changes in Chemical Composition of Living Cells

We present a method for direct non-optical quantification of dry mass, dry density and water mass of single living cells in suspension. Dry mass and dry density are obtained simultaneously by measuring a cell’s buoyant mass sequentially in an H(2)O-based fluid and a D(2)O-based fluid. Rapid exchange...

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Main Authors: Feijó Delgado, Francisco, Cermak, Nathan, Hecht, Vivian C., Son, Sungmin, Li, Yingzhong, Knudsen, Scott M., Olcum, Selim, Higgins, John M., Chen, Jianzhu, Grover, William H., Manalis, Scott R.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Public Library of Science 2013
Ábhair:
Research Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3699654/
https://ncbi.nlm.nih.gov/pubmed/23844039
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0067590
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