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A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.

This paper describes a plasmid vector for cloning cDNAs in Escherichia coli; the same vector also promotes expression of the cDNA segment in mammalian cells. Simian virus 40 (SV40)-derived DNA segments are arrayed in the pcD vector to permit transcription, splicing, and polyadenylation of the cloned...

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Main Authors: Okayama, H, Berg, P
格式: Artigo
語言:Inglês
出版: 1983
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC368532/
https://ncbi.nlm.nih.gov/pubmed/6300662
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